流感A+B病毒診斷試劑（FUJIREBIO）

廣州健侖生物科技有限公司

廣州健侖長期供應(yīng)各種流感檢測(cè)試劑，包括進(jìn)口和國產(chǎn)的品牌，主要包括日本富士瑞必歐、日本生研、美國BD、美國NovaBios、美國binaxNOW、日本積水、日本榮研、美國OSOM、芬蘭、愛思普林、英國Clearview、凱必利、廣州創(chuàng)侖等主流品牌。

流感A+B病毒診斷試劑（FUJIREBIO）

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

（3）DAB變質(zhì)和顯色時(shí)間太長：DAB現(xiàn)用現(xiàn)配，如有沉渣應(yīng)進(jìn)行過濾后再用。配制好的DAB不應(yīng)存放時(shí)間太長，因?yàn)樵跊]有酶的情況下，過氧化氫也會(huì)游離出氧原子與DAB產(chǎn)生反應(yīng)而降低DAB的效力，未用完的DAB存放在冰箱里幾天后再用這種似乎節(jié)約的辦法是不可取的。DAB的顯色在顯微鏡下監(jiān)控，達(dá)到理想的染色程度時(shí)立即終止反應(yīng)。不過當(dāng)染色片太多時(shí)或用染色機(jī)時(shí)，這樣做似乎不現(xiàn)實(shí)，但至少應(yīng)對(duì)一些新的或少用的抗體顯色時(shí)進(jìn)行監(jiān)控，避免顯色時(shí)間過長。
（4）組織變干：修復(fù)液溢出后未及時(shí)補(bǔ)充液體、染色切片太多、動(dòng)作太慢、忘記滴液、滴液流失等都是造成組織變干的原因。解決的辦法是操作要認(rèn)真仔細(xì)，采用DAKO筆或PAP Pen在組織周圍畫圈，可以有效的避免液體流失，也能提高操作速度。
（5）切片在緩沖液或修復(fù)液中浸泡時(shí)間太長（大于24小時(shí)）：原因上不清楚，但現(xiàn)象存在。有的實(shí)驗(yàn)室喜歡前一天將切片脫蠟至修復(fù)，第二天加抗體進(jìn)行免疫組化染色，如果將裝有切片和修復(fù)液的容器放在4oC冰箱過夜，對(duì)結(jié)果無明顯影響，如果放在室溫，特別是炎熱的夏天，會(huì)出現(xiàn)背景著色，因此，不可存放時(shí)間太長。
（6）一抗變質(zhì)、質(zhì)量差的多克隆抗體：注意抗體的有效期，過期的抗體要麼不顯色要麼背景著色。用新買的抗體時(shí)設(shè)立陽性對(duì)照和用使用過的抗體作比較。

(3) DAB metamorphism and color development time is too long: DAB is best used now with sediment should be filtered before reuse. DAB prepared should not be stored for too long, because in the absence of enzymes, hydrogen peroxide will also free oxygen atoms react with DAB to reduce the effectiveness of DAB, DAB is not exhausted in the refrigerator a few days later Reusing this seemingly economical approach is not desirable. The color of DAB is best monitored under a microscope, and the reaction is stopped immediay when the desired degree of staining is achieved. However, this may not be the case when there are too many dyed sheets or when using a dyeing machine, but at least some new or less used antibodies should be monitored during their color development to avoid excessive color development.
(4) tissue dry: repair fluid overflow after the liquid is not replenished in time, too many dyed slices, the action is too slow, forget the drop, drip loss and so are the causes of tissue dryness. The solution is to be careful operation, the use of DAKO pen or PAP Pen circle around the organization, can effectively avoid the loss of liquid, but also improve operating speed.
(5) Slides immersed in buffer or repair fluid too long (more than 24 hours): the reason is unclear, but the phenomenon exists. Some labs like to deparaffinize the sections the day before to repair, and the next day add antibodies for immunohistochemical staining. If a container with sections and reconstitution fluid is placed in a 4oC refrigerator overnight, there is no significant effect on the results. If placed in Room temperature, especially hot summer, there will be background coloring, therefore, can not be stored for too long.
(6) First-class anti-aging, poor quality polyclonal antibodies: pay attention to the validity of antibodies, expired antibodies either not color or background coloring. It is best to set up a positive control with the newly purchased antibody and compare with the used antibody.