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麻疹病毒IgG ELISA檢測試劑盒

麻疹病毒IgG ELISA檢測試劑盒

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麻疹病毒IgG ELISA檢測試劑盒:麻疹是兒童Z常見的急性呼吸道傳染病之一,其傳染性很強,在人口密集而未普種疫苗的地區易發生流行,2~3年一次大流行。麻疹病毒屬副黏液病毒,通過呼吸道分泌物飛沫傳播。如需了解詳情請廣州健侖生物科技有限公司

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麻疹病毒IgG ELISA檢測試劑盒

英文名稱:Measles virus IgG enzyme-linked immunosorbent assay kit

廣州健侖生物科技有限公司

 

廣州健侖生物科技有限公司是集研制開發、銷售、服務于一體的優良企業,公司產品涉及臨床快速診斷試劑、食品安全檢測試劑,違禁品快速檢測,動物疾病防疫檢測試劑,免疫診斷試劑、臨床血液學和體液學檢驗試劑、微生物檢驗試劑、分子生物學檢驗試劑、臨床生化試劑、有機試劑等眾多領域,同時核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名診斷產品集團公司產品,致力于為商檢單位、疾病預防控制中心、海關出入境檢疫局、衛生防疫單位,緝毒系統,戒毒中心,檢驗檢疫單位、生化企業、科研院所、醫療機構等機構與行業提供*、高品質的產品服務。此外,本公司還開展食品、衛生、環境、藥品等多方面的第三方檢測服務。

主要用途:用于定量測定人血清、腦脊液或血漿中的麻疹抗體。

產品規格:96T/盒

存儲條件:4-8

保質期:18個月

我司同時還提供美國FOCUS、西班牙DIA美國trinity試劑盒:

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【產品介紹】

(1)潛伏期約10日(6~18天)。曾經接觸過麻疹患兒或在潛伏期接受被動免疫者,可延至3-4周。在潛伏期內可有輕度體溫上升。

(2)前驅期也稱發疹前期,一般為3~4天。表現類似上呼吸道感染癥狀:①發熱見于所有病例,多為中度以上發熱;②咳嗽、流涕、流淚、咽部充血等,以眼癥狀突出,結膜發炎、眼瞼水腫、眼淚增多、畏光、下眼瞼邊緣有一條明顯充血橫線(Stimson線),對診斷麻疹極有幫助。③麻疹黏膜斑,在發疹前24~48小時出現,為直徑約1.0mm灰白色小點,外有紅色暈圈,開始僅見于對著下臼齒的頰黏膜上,但在一天內很快增多,可累及整個頰黏膜并蔓延至唇部黏膜,黏膜疹在皮疹出現后即逐漸消失可留有暗紅色小點;④偶見皮膚蕁麻疹,隱約斑疹或猩紅熱樣皮疹,在出現典型皮疹時消失;⑤部分病例可有一些非特異癥狀,如全身不適、食欲減退、精神不振等。但體溫稍有下降。嬰兒可有消化系統癥狀,嘔吐、腹瀉等。

(3)出疹期多在發熱后3-4天出現皮疹。體溫可突然升高至40~40.5℃,皮疹為稀疏不規則的紅色斑丘疹,疹間皮膚正常,出疹順序也有特點:始見于耳后、頸部、沿著發際邊緣,24小時內向下發展,遍及面部、軀干及上肢,第3天皮疹累及下肢及足部。病情嚴重者皮疹常融合,皮膚水腫,面部水腫變形。大部分皮疹壓之褪色,但亦有出現淤點者。全身有淋巴結腫大和脾腫大,并持續幾周,腸系膜淋巴結腫大可引起腹痛、腹瀉和嘔吐。闌尾黏膜的麻疹病理改變可引起闌尾炎癥狀。疾病極期特別是高熱時常有譫妄、激惹及嗜睡狀態,多為一過性,熱退后消失,與以后中樞神經系統合并癥無關。此期肺部有濕性羅音,X線檢查可見肺紋理增多。

 

(1) incubation period of about 10 days (6 to 18 days). Those who have been exposed to measles or passive immunization in the incubation period may be extended to 3-4 weeks. In the incubation period may have mild body temperature rise.

(2) Precursor Period also known as pre-eruption, usually 3 to 4 days. Symptoms similar to the upper respiratory tract infection: fever found in all cases, mostly moderate to moderate fever; cough, runny nose, tears, throat congestion, prominent in ocular symptoms, conjunctival inflammation, eyelid edema, increased tear, photophobia, The lower eyelid margin has an obvious congestion line (Stimson line), extremely helpful in the diagnosis of measles. Measles mucosa spots, 24 to 48 hours before the onset of rash appeared as a diameter of about 1.0mm gray dots, a red halo outside, beginning only seen in the buccal mucosa facing the lower molars, but soon increased in one day, Can affect the entire buccal mucosa and spread to the lip mucosa, mucosal rash disappears after the rash may appear dark red dots can be left; ④ occasional skin urticaria, vague rash or scarlet fever-like rash disappears in the presence of a typical rash ; ⑤ some cases may have some non-specific symptoms, such as general malaise, loss of appetite, lack of energy and so on. But the temperature dropped slightly. Infants may have digestive symptoms, vomiting, diarrhea and more.

(3) rash more than 3-4 days after the onset of fever rash. Body temperature can suddenly rise to 40 ~ 40.5 ℃, the rash is sparse irregular red rash, rash skin is normal, the order of the rash also has characteristics: first seen in the ear, neck, along the hairline edge, down within 24 hours Development, throughout the face, trunk and upper extremities, the third day of the rash involving the lower extremities and feet. Patients with severe rash often confluence, skin edema, facial edema deformation. Most of the skin rash pressure fade, but there are also those who have spots. Systemic lymph nodes and splenomegaly, and for several weeks, mesenteric lymph nodes can cause abdominal pain, diarrhea and vomiting. Appendectomy pathological changes in measles can cause appendicitis symptoms. Extreme disease especially during high fever, often delirium, irritability and lethargy state, mostly transient, disappear after the heat back and forth, has nothing to do with the subsequent central nervous system complications. This period of lung wet rales, X-ray examination showed increased lung texture.

【檢測原理】

ELISA(酶聯免疫吸附測定)是涉及的免疫學過程在抗體檢測的感染領域尤其得到證實。該基于抗體和抗原的特異性相互作用的檢測反應。至為此目的,使用賽潤ELISA classic的微量滴定板的測試條傳染性病原體特異性抗原在患者樣品中的結合包被的抗體存在。 其他用堿性磷酸酶標記二抗檢測由此形成的免疫復合物。 該酶催化a反應過程中,無色底物對硝基苯磷酸酯在有色產物中對硝基苯酚轉化。 反應產物的信號強度正比于樣品中的抗體濃度用光度法檢測。

麻疹病毒IgG ELISA檢測試劑盒

【試劑盒的組成】

試劑盒組成

IgG試劑盒  IgM試劑盒 IgA試劑盒  

數量 / 容積 

微孔條(此微孔條可拆下單獨使用,每條有8孔,共96孔,已經包被了抗原) 

1個微孔條框架 

包被材料未被激活 

12           12          12

標準血清(立即可用) 

人血清溶于含蛋白的磷酸鹽緩沖液;抗HIV抗體、抗乙肝病毒(HBV)表面抗原和抗丙肝病毒(HCV)抗體均為陰性; 

防腐劑:< 0.1% * 

染色劑:紫紅色O

2×2毫升    2×2毫升   2×2毫升

陰性對照血清(立即可用) 

人血清溶于含蛋白的磷酸鹽緩沖液;抗HIV抗體、抗乙肝病毒(HBV)表面抗原和抗丙肝病毒(HCV)抗體均為陰性; 

防腐劑:< 0.1% * 

染色劑:里沙明綠 V

1×2毫升   1×2毫升    1×2毫升

酶標記的抗人IgG, IgA, IgM (立即可用) 

羊抗人IgG, IgA, IgM(多克隆),標記堿性磷酸酶后在蛋白穩定劑中儲存 

防腐劑: 0.01% 甲基異噻唑啉酮 

 0.01% 溴化硝基二堊烷 

13毫升      13毫升      13毫升 

濃縮洗液(可稀釋至1000毫升) 

氯化鈉溶液,含吐溫2030mM Tris  

防腐劑: < 0.1%* 

1×33.3毫升  1×33.3毫升 1×33.3毫升 

稀釋緩沖液 

磷酸鹽緩沖液,內含蛋白和吐溫20

防腐劑: < 0.1%* 

0.01 /升的溴酚藍鈉鹽 

2×50毫升   2×50毫升 2×50毫升

終止液 

1.2N 氫氧化鈉 

15毫升      15毫升      15毫升 

底物(立即可用) 

對硝基苯磷酸鹽,不含其它溶劑的緩沖液 

防腐劑:< 0.1% * 

(未開封瓶子中的底物可能會輕微變黃,但不會影響其質量) 

13毫升      13毫升      13毫升 

帶有標準曲線和評估表的質量控制文件 

(抗體以IU/毫升或U/毫升計量) 

1             1           1 

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不受管制而迅速增殖的細胞通常會轉變成良性腫瘤或惡性腫瘤。良性腫瘤不會擴散到身體其他部份,或是侵入別的組織,除非腫瘤的生長壓迫到重要的器官,否則也不會影響生命。惡性腫瘤則會侵略其他器官,轉移到身體其他部位而危害生命。
引起基因突變的物質被稱為致癌物質,又以其造成基因損傷的方式可分為化學性致癌物與物理性致癌物。例如接觸放射性物質,或是一些環境因子,例如,香煙、輻射、酒精。還有一些病毒可將本身的基因插入細胞的基因里中,激活癌基因。但突變也會自然產生,所以即使避免接觸上述的致癌因子,仍然無法*預防癌癥的產生。發生在生殖細胞的突變有可能傳至下一代。
現知有1000多種化學物質具致癌作用,如黃曲霉素可致肝癌,香煙的燃燒物可致肺癌,亞硝胺可致食道癌。物理致癌因素中,放射線可致白血病,過強紫外照射和局部慢性刺激可致皮膚癌。病毒、寄生蟲感染等生物因素可能與鼻咽癌、肝癌有一定關系。家族遺傳、免疫水平、精神刺激、營養狀態以及內分泌活動等因素亦對腫瘤的發生起重要作用。尤其是精神因素與癌癥的近來倍受重視。
生活方式的影響
生活方式對于癌癥發生確實是有影響,例如香煙、飲食、運動、酒精、曬太陽以及性病等。
zui近幾十年的研究中,zui為確定的發現就是抽煙和癌癥間密切的相關性,許多流行病學研究也已經證實這樣的關系。根據美國的數據顯示,隨著吸煙人數增加,肺癌的死亡率急劇升高。
同時有越來越多的研究顯示,癌癥發生也和體內褪黑激素的量相關,當需要長時間待在明亮的環境下,例如晚班的工人。或是睡眠時間較短的人,褪黑激素表現量也會偏低,而癌癥的發病率較高。
癌癥的傳染
有些并非發生在人類的癌癥可能能經由傳染而引起,例如發生于狗的史狄可氏肉瘤(Sticker's sarcoma)。有病患接受細菌,由于移植器官中帶有腫瘤,結果得到癌癥。這是目前已知較類似經由傳染而得的例子。
癌癥的遺傳

Unregulated and rapidly proliferating cells often turn into benign or malignant tumors. Benign tumors do not spread to other parts of the body, or invade other tissues, and do not affect life unless the growth of the tumor compresses into vital organs. Malignant tumors will invade other organs, transfer to other parts of the body and endanger life.
Substance causing mutations are called carcinogens, but also its way to cause genetic damage can be divided into chemical carcinogens and physical carcinogens. Such as exposure to radioactive material, or some environmental factors, such as cigarettes, radiation, and alcohol. Still others can insert their own genes into the cell's genes and activate oncogenes. But mutations also occur naturally, so even if you avoid contact with these oncogenic factors, you still can not compley prevent cancer. Mutations that occur in germ cells are likely to reach the next generation.
Now known to have more than 1,000 kinds of chemical substances with carcinogenic effects, such as aflatoxin can cause liver cancer, the combustion of cigarettes can cause lung cancer, nitrosamines can cause esophageal cancer. Among the physical carcinogenic factors, radiation can cause leukemia, too strong UV irradiation and local chronic irritation can cause skin cancer. Viruses, parasitic infections and other biological factors may be related to nasopharyngeal carcinoma, liver cancer have a certain relationship. Family genetic, immune levels, mental stimulation, nutritional status and endocrine activities and other factors also play an important role in tumorigenesis. In particular, the link between mental factors and cancer has drawn much attention recently.
The impact of lifestyle
Lifestyles do have an impact on cancer, such as cigarettes, diet, exercise, alcohol, sunbathing, and sexually transmitted diseases.
The most definitive finding in recent decades of research is the close correlation between smoking and cancer, and many epidemiological studies have confirmed this relationship. According to the U.S. data, as the number of smokers increases, the mortality rate of lung cancer has risen sharply.
In the meantime, more and more studies show that the incidence of cancer is also related to the amount of melatonin in the body when it takes a long time to stay in a bright environment, such as night shift workers. Or people who sleep for a short time, melatonin performance will be low, while the incidence of cancer is higher.
Infection with cancer
Some cancers that are not in humans can be caused by infection, such as Sticker's sarcoma in dogs. Patients receive the bacteria, and as a result of the tumor in the transplanted organ, the result is cancer. This is an example known to be more contagious now.
Genetics of cancer

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