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美國NOVABIOS乙型腦炎病毒檢測試劑盒

美國NOVABIOS乙型腦炎病毒檢測試劑盒

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美國NOVABIOS乙型腦炎病毒檢測試劑盒用于暴露于日本腦炎病毒(JEV)是用于檢測人血清中的IgE抗體對JEV衍生的重組抗原(JERA)(1-4)的ELISA測定系統。這個測試是為了幫助診斷人類暴露于日本腦炎病毒(JEV)。它不是為了篩選血液或血液成分,僅用于專業的體外診斷用途。該試劑盒尚未針對疫苗誘導的血清學轉換研究進行優化。

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美國NOVABIOS乙型腦炎病毒檢測試劑盒

 

  (我司還提供瘧疾、登革熱、基孔肯雅熱、克維錐蟲、RK39和黃熱病等檢測試劑盒,咨詢)

JE DetectTM IgM ANTIBODY

CAPTURE ELISA (MAC-ELISA)

JE Detect MAC-ELISA-specific materials:

 

  • Anti-Human IgM Coated Microtiter Strips: Strip holder with cover, containing 96 polystyrene

美國NOVABIOS乙型腦炎病毒檢測試劑盒

microtiter wells coated with antibody to human IgM in each well. Store at 2-8ºC until ready to use. The anti-human IgM coated wells are used to capture IgM antibodies from human samples.

  • Sample Dilution Buffer for JE DetectIgM: One bottle, 25 mL, for ELISA plate wells in IgM assay. Store at –2-8º C until ready to use.

 

Note: If any precipitate is seen, vortex the tube very well to obtain a homogeneous solution and then use.

 

  • Ready-to-use JE Antigen (JERA) for IgM: One tube (3 ml) of Ready-to-use JERA solution. Store at

–2-8º C until ready to use.

  • Ready-to-use normal cell antigen (NCA) for IgM: One tube (3 ml) of Ready-to-use NCA solution. Store at –2-8º C until ready to use.
  • JE Detect Negative Control: One vial, 30 µL of heat–inactivated serum. The JE Detect Negative Control will aid in monitoring the integrity of the kit as well. Store at 2-8°C until ready to use for up to 7 days. Quick spin the vial briefly before use to collect the content at the bottom.

Note: For long-term storage, serum can be further aliquoted in a smaller volume and stored at –70°C.

  • JE Detect IgM Positive Control: One vial, 30 µL of heat–inactivated serum. The JE Detect IgM Positive Control will aid in monitoring the integrity of the kit as well. Store at 2-8°C until ready to use for up to 7 days. Quick spin the vial briefly before use to collect the content at the bottom.

Note: For long-term storage, serum can be further aliquoted in a smaller volume and stored at –70°C.

  • 10X Wash Buffer: One bottle, 120 mL of Wash Buffer to be used in all the washing steps of this procedure. Store at 2-8°C until ready to use.
  • Ready to Use Enzyme Conjugate-HRP for IgM: One bottle, 6 mL of a pre-diluted HRP conjugated flavivirus reactive monoclonal antibody (mAb) to be used as is in the procedure below. Store at 2-8°C until ready to use.
  • EnWash: One bottle, 20 mL of EnWash to be used in between the washing steps after the addition of enzyme conjugate-HRP of this procedure. Store at 2-8º C until ready to use.

 

  • Liquid TMB Substrate: One bottle, 9 mL of liquid substrate to be used in this procedure. Store at 2-8ºC until ready to use.

 

Note: The substrate should be kept in a light -protected bottle at all times as provided.

11. Stop Solution: One bottle, 6 mL to be used to stop the reaction. Store at 2-8°C until ready to use.

 

Caution: strong acid, wear protective gloves, mask and safety glasses. Dispose of all materials according to safety rules and regulations.

 

更具體的說明請根據以下信息

 

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